A Deinococcus radiodurans Nudix fehérjéjének klónozása, expressziója és enzimaktivitásának mérése
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The aim of my work was the experimental examination of the disordered regions to verify my hypothesis. For this it was necessary to produce the wild-type Nudix DR0550 protein. I cloned the DNA region coding for the enzyme, cloned it into an expression vector, purified the protein and measured the enzyme activity. I worked with the wild-type as well as with the Nudix-dN mutant which is truncated at its N-terminal, lacking the longer disordered region.