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Benzimidazol-rezisztencia kimutatása PCR–RFLP-módszerrel juhból izolált Haemonchus contortusban - Esetismertetés
dc.contributor.authorNagy, Gábor
dc.contributor.authorZsolnai, Attila
dc.contributor.authorCsivincsik, Ágnes
dc.contributor.authorSugár, László
dc.date.accessioned2021-04-30T12:46:59Z
dc.date.available2021-04-30T12:46:59Z
dc.date.issued2015-03
dc.identifier.citationMagyar Állatorvosok Lapja 137(3), 167-172. (2015)en_US
dc.identifier.urihttp://hdl.handle.net/10832/2919
dc.description.abstractSummary The authors dissected a ewe died of haemonchosis on the 26th day after an albendazole treatment. The large number of Haemonchus contortus recovered from the abomasum suggested the possibility of benzimidazole resistance, which was confirmed by PCR–RFLP based on single-nucleotide polimorphism of codon 200 in isotype 1 ß-tubulin gene (Phe200Tyr). The proportion of resistant homozygotes (RR), heterozygotes (RS) and susceptible homozygotes (SS) were 66.78%, 33.33% and 0%, respectively (n = 15). By Haldane’s exact test Hardy–Weinberg equilibrum of the population was accepted (p = 1.0). The true prevalence of resistant genotype (RR), which was calculated by Sterne’s exact method, proved to be 39.68–85.83% (confidence interval: 95%). These results confirmed that long-term usage of benzimidazoles can change the relative allele frequency of genes associated with drug resistance; hereby it can cause large-scale spread of resistant allele. To our knowledge, this study proved the resistance to benzimidazole in Haemonchus contortus in Hungary for the first time.en_US
dc.language.isohuen_US
dc.publisherMagyar Állatorvosok Lapjaen_US
dc.titleBenzimidazol-rezisztencia kimutatása PCR–RFLP-módszerrel juhból izolált Haemonchus contortusban - Esetismertetésen_US
dc.title.alternativeDetection of benzimidazole resistance by PCR–RFLP in Haemonchus contortus recovered from sheep - Case reporten_US
dc.typeArticleen_US
dcterms.bibliographicCitationMagyar Állatorvosok Lapja 137(3), 167-172. (2015)


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