Title:Citrobacter freundii okozta vérfertőzés újszülött borjúban Esetismertetés
Lipovszky, András Dénes
SUMMARY Background: Septicaemia is a dangerous often fatal disease of new-born calf, which is induced by Escherichia coli bacterium in majority of the cases. Objectives: The authors report the first case of Citrobacter freundii induced sep ticaemia in a new-born calf. Materials and Methods: Two calves died in a small herd presenting weakness and anorexia in age of 2 days. One of these animals was presented for laboratory examination. After gross pathological investigation bacteriological examinations were performed from the joint, lungs and liver. The disk diffusion test was used for antibiotic sensitivity of the isolated bacterium strain. Tissue samples were col lected from brain, lungs, spleen, liver, and kidney for histological and immunohis tochemical (IHC) examinations. A rabbit anti-Mycobacterium bovis (BCG) antibody was used for IHC test, which detects several different bacterial species. Results and Discussion: Mild icterus and suffusion haemorrhages on serous membranes were observed. Histological examination revealed acute haemor rhages in meninges, kidney, spleen and lungs. Serous meningitis, acute degen eration of liver and kidney and mild interstitial pneumonia was also present. Additionally coccobacilli were found in the brain, lungs, kidney, and spleen with histological examination and with IHC methods. Coliform bacterium was cultured from lungs and liver, which was identified as C. freundii. The strain was sensitive to gentamycin, streptomycin, colistin, marbofloxacin, tetracycline, doxycycline, sulphonamide+trimethoprim, ceftiofur and cefquinome. The bacterium strain was moderate resistant to neomycin, spectinomycin, enrofloxacin, flumequine, florfenicol, and it was resistant to ampicillin, amoxicillin, amoxicillin+clavulanic acid, sulphonamide and cephalexin. Other bacteria (including Brucella and Cam pylobacter) or viruses (including Schmallenberg virus, bluetongue virus and bovine virus diarrhoea virus) were not detected.