Hungarian Veterinary Archive

HomeCollectionsKnowledge baseSearch/BrowseConnection
JavaScript is disabled for your browser. Some features of this site may not work without it.
Title:
Effect of Salmonid alphavirus infection on housekeeping gene expression, validation by quantitative, real-time PCR
Author:
Kverneland, Elisabeth
Date:
2017
Abstract:
Pancreas disease is responsible for major economic losses in the European salmonid farming industry, due to mortality, diminished growth rate and reduced slaughter weights. The infectious agent causing pancreas disease in Atlantic salmon was first isolated in Scotland in 1995 and later classified as an Alphavirus, the first Alphavirus to be isolated that affects fish. One hallmark of Alphavirus infection in vertebrate cells is the ability of the pathogen to shut down host transcription and translation processes without affecting viral protein and nucleic acid synthesis. It is of interest to find out if SAV shares this characteristic. The goal of this project was to investigate Salmonid alphaviruses ability to down-regulate host cell transcription. Through using a two-step quantitative PCR for analysis of different housekeeping genes in non-infected and infected cells, we hoped to evaluate if SAV3 causes down-regulation of transcription. If so, at what time interval it occurred, and to what degree. This study and its incurred results served as a pilot study, to optimize and fine-tune the methodology for further experimentation.
URI:
http://hdl.handle.net/10832/1758
Elisabeth Kverneland Thesis (1.883Mb)
Share:
gffdg
Share on Google+
Show full item record

 

 

magyarenglish

Search

Go to advanced search

Login

Open Archives InitiativeMagyar Tudományos Művek Tára